IBSG Webinar 10-2021: Chromatin Remodeling Complexes

Kính mời quý độc giả tham dự buổi Webinar tháng 10-2021 do Nhóm Học Thuật Y Sinh IBSG tổ chức trực tuyến qua Zoom.

Trong buổi webinar chuyên đề này, IBSG hân hạnh được sự tham gia của:

T.s. Đào Thanh Hải: Princeton University, USA; Incoming assistant faculty at St. Jude Children’s Research Hospital.

Chủ đề: Elucidating the functions of the ATP-dependent chromatin remodeling complexes with chemical biology.

Host: Nguyễn Bích Nụ

Abstract:

Regulation of chromatin landscape is intimately linked with DNA-templated processes such as transcription, replication, and DNA damage repair. Aberrant chromatin regulation can serve as an underlying cause of genetic disorders and cancers. The ATP-dependent chromatin remodeling complexes (remodelers) are multicomponent chromatin regulators that play critical roles in establishing and modulating genomic architecture. These megadalton-sized molecular machines are mutated in over 20% of malignancies, underscoring their functions in gene expression. It is known that remodelers can directly interact with multiple histone domains of the nucleosome to elicit their functions. However, the specific histone features that govern their binding and activities remain undefined. I will discuss how chemistry-based approaches can help elucidate the molecular interplays between chromatin and chromatin remodeling complexes by giving you three examples. First, I will describe a photocrosslinking-MS-based technology (‘photoscanning’) for profiling protein-histone interaction and its applications to identify unknown histone interacting domains of the ISWI and SWI/SNF remodelers. Next, I will discuss how cancer-associated histone mutations disrupt the directionality of chromatin remodeling based on remodeling assays with novel nucleosome substrates. Finally, I will discuss a high-throughput screening platform (termed DNA-barcoded nucleosome library) for studying the effect of histone PTMs on the binding and activity of the SWI/SNF remodelers.

Thời gian: 9:00 – 10:00 AM Chủ Nhật giờ Việt Nam (tương ứng với 7:00 – 8:00 PM thứ Bảy theo giờ California), ngày 17.10.2021.

Tham dự trực tuyến qua Zoom tại: https://uwmadison.zoom.us/j/97899997972

Meeting ID: 978 9999 7972

Lệ phí tham dự: hoàn toàn miễn phí

Lưu ý: Khách tham dự không ghi âm, quay clip, và chụp hình khi chưa có sự đồng ý của diễn giả (ban tổ chức sẽ phụ trách việc này).