Học bổng PhD sinh học thực vật làm việc ở Pháp và Áo 2019

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This PhD subject will be directed by S. Hawkins (Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) UMR CNRS 8576, Université de Lille, France) and N. Gierlinger (Institute for Biophysics, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria). The candidate selected will spend 18 months (approximately) in France, and 18 months in Austria.

Proposition of a co-tutored (France-Austria) PhD thesis financed on regional and European funds, start date October 2019

“Characterization of the spatial distribution of lignin in plant cell walls using chemical reporters and Raman spectroscopy”

The objectives of this PhD thesis co-directed by the Dr Notburga Gierlinger (BOKU, Austria) and the Pr Simon Hawkins (CNRS-University of Lille, France) are to improve our understanding of the spatial distribution of lignin within the cell walls of several different plant species (model species and species of economic interest: flax, hemp, poplar, spruce, maize, Brachypodium).

Lignin plays a major role in the growth and development of plants, and contributes to their defense against abiotic stress and pathogens. In an applied context, the quantity of lignin, its biochemical composition, but also its distribution within the different layers of the cell wall, strongly affect the quality of several plant-derived industrial products. In this PhD we will use two complementary and powerful imaging techniques to map the spatial distribution of lignin in the cell wall during its in planta assembly and during degradation (enzymatic/chemical). The first technique, developed by the teams of Simon Hawkins and Christophe Biot (CNRS-University of Lille), relies upon the use of chemical reporters to mark the lignin polymer 1. The second strategy, exploited by Dr Notburga Gierlinger (BOKU, Austria) is based upon the use of Raman vibrational spectroscopy 2. The data generated by the two approaches will be combined to provide detailed information about the spatial distribution of lignin within the cell wall. In collaboration with different INRA teams, we will map the spatial distribution of cell wall lignin in several maize genotypes and compare it to observed differences in digestibility in order to demonstrate the applied interest of using imaging techniques for analyzing the physic-chemical behavior of plant biomass.

Publications

  1. Simon, C., Lion, C., Spriet, C., Baldacci-Cresp, F., Hawkins, S., & Biot, C. (2018). One, two, three: a bioorthogonal triple labelling strategy for studying the dynamics of plant cell wall formation in vivo. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201808493
  2. Gierlinger, N. (2014). Revealing changes in molecular composition of plant cell walls on the micron-level by Raman mapping and vertex component analysis (VCA). Frontiers in plant science, 5, 306.
  3. Simon C, Lion C, Biot C, Gierlinger N and Hawkins S. (2018). Lignification and advances in lignin imaging in plant cell walls. Ann. Plant Rev. online. https://doi.org/10.1002/9781119312994.apr0656
  4. Özparpucu, M., Rüggeberg, M., Gierlinger, N., Cesarino, I., Vanholme, R., Boerjan, W., & Burgert, I. (2017). Unravelling the impact of lignin on cell wall mechanics: a comprehensive study on young poplar trees downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD). The Plant Journal, 91(3), 480-49

Contacts:

Simon HAWKINS: simon.hawkins@univ-lille.fr; +33 (0)3 20 43 40 30

Notburga GIERLINGER: burgi.gierlinger@boku.ac.at;

Nguồn: Dr. Nguyễn Tấn Trung

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